Browsing by Author "MAHMOUD, Salma Abdi"
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- Item8-Hydroxy-2'-Deoxyguanosine (8-OH-2dG) as a Biomarker of Oxidative Stress (OS) in the Acute Exacerbation of Spontaneous Preterm Birth (SPTB)/(Scientific Reseach Publishing, 2024-10-12) MAHMOUD, Salma AbdiSpontaneous preterm birth (SPTB) is characterized by the delivery of a baby before 37 completed weeks of gestation, and this condition is associated with significant health challenges for the newborn. Emerging evidence highlights the importance of biomarkers for understanding the mechanisms underlying SPTB. One such biomarker, 8-OH-2dG, plays a critical role in evaluating oxidative stress and its impact on pregnancy outcomes. It has been demonstrated that 8-OH-2dG is a product of oxidative DNA damage and is widely recognized as a key indicator of cellular oxidative stress. Elevated reactive oxygen species in SPTB result in higher levels of the DNA degradation product 8-OH- 2dG in amniotic fluid, causing damage to motoneuronal and fetal tissues that could lead to premature rupture of fetal membranes. Therefore, evaluating the role of 8-OH-2dG in SPTB is of great interest. This review provides an overview of the current knowledge on 8-OH-2dG as a biomarker for SPTB and aims to elucidate its mechanism in this condition.
- ItemIntermittent Fasting (IF) Reduces Tumor Metastasis via Ras/MAPK, PI3K/AKT/mTOR, Wnt/β-Catenin, and HGF/c-Met Pathways(Open Journal of Obstetrics and Gynecology / Scientific Reseach Publishing, 2024-12-19) MAHMOUD, Salma AbdiSeveral recent scientific interventions have been conducted to investigate the effects of intermittent fasting (IF) on tumor metastasis. It is well known that IF has a positive effect on reducing OS in the human body. OS is an important factor that leads to DNA damage and stimulates carcinogenesis through dysregulation of signaling pathways that are important for tumor survival and metastasis. Studies have demonstrated that mitogen-activated protein kinase (Ras/MAPK), phosphatidylinositol- 3-kinase/mammalian target of rapamycin (PI3K/AKT/mTOR), Wnt Beta Catenin (Wnt/β-catenin), and hepatocyte growth actor/mesenchymal- epithelial transition factor (HGF/c-Met) are activated in response to the overproduction of OS and may result in carcinogenesis and tumor metastasis. In this review, we discuss the regulatory mechanism of IF in tumor metastasis by downregulating key OS pathways such as Ras/Raf/MAPK, PI3K/AKT/mTOR, Wnt/β-catenin, and HGF/c-Met.
- ItemOver expression of sulfatase-1 in murine hepatocarcinoma Hca-F cell line downregulates mesothelin and leads to reduction in lymphatic metastasis, both in vitro and in vivo(Oncotarget,, 2016-11-10) MAHMOUD, Salma AbdiLymphatic vessels function as transport channels for tumor cells to metastasize from the primary site into the lymph nodes. In this experiment we evaluated the effect of Sulfatase-1 (Sulf-1) on metastasis by upregulating it in murine hepatocarcinoma cell line Hca-F with high lymph node metastatic rate of >75%. The study in vitro showed that up regulation of Sulf-1 in Hca-F cells significantly reduced cell proliferation, migration and invasion (p<0.05). Also, the forced expression of Sulf-1 down regulated Mesothelin (Msln) at both the protein and mRNA levels. The experiment in vivo further showed that up-regulation of Sulf-1 with the attendant downregulation of mesothelin delayed tumor growth and decreased lymph node metastasis. In conclusion, our findings show that Sulf-1 is an important tumor suppressor gene in hepatocellular carcinoma (HCC), and its over expression downregulates Msln and results in a decrease in HCC cell proliferation, migration, invasion, and lymphatic metastasis. This functional relationship between Sulf-1 and Msln could be exploited for the development of a novel liver cancer therapy
- ItemPrevalence and Factors Associated with Non Adherence to Iron and Folic Acid Supplementation among Women for Antenatal Care at Mwembeladu Hospital, Zanzibar(Journal of Pharmaceutical Research International, 2024-08-16) Chukwuma J. Okafor a*, Rodolfo Isidro Bosch Bayard b, Diane Millo Martin c, Salma Mahmoud d, Said A. Yusuf e, Minja T. Lilian f, E N Adejumo g, Nibras M. Hilal b, Tarik A. Khamis b, Asila M. Haji b and Rayan J. Ali b; MAHMOUD, Salma AbdiBackground: Daily iron and folic acid supplementation (IFAS) during pregnancy reduces the risk of all types of maternal anaemia and iron deficiency anaemia at term; despite the WHO recommendations, pregnant women are still vulnerable because the use of Iron and Folic Acid Supplementation is still low in many countries including Tanzania. Therefore, the study aims to comprehensively understand the prevalence and factors associated with non-adherence to Iron and Folic Acid Supplementation among pregnant women attending Antenatal Care at Mwembeladu Hospital. Achieving this objective will contribute to developing effective strategies to improve IFAS adherence. Methodology: The study was cross-sectional and ANC-based, using questionnaires. Questionnaires were distributed to the 260 pregnant women attending ANC at Mwembeladu Hospital. Data were analyzed using SPSS computer software version 22. Results: Our study showed that 52.7% had more than 4 ANC visits. Based on self-reported adherence, about (61.9%) of pregnant women were taking IFAS supplementation, and (38.1%) were not taking IFAS completely. Among those taking IFAS, (60.2%) out of 161 pregnant women were taking four tablets per week as recommended by WHO, and (39.8%) out of 161 were not following the WHO recommendations. Therefore, out of 260, zero adherence was 38.1%, poor adherence was 24.6%, and good adherence was 37.3%. Hence, 62.7% were non-adherent to IFAS supplementation as recommended by WHO. Also, the result showed that the major factors militating against pregnant women taking IFAS are lack of knowledge of IFAS (22.1%), side effects of IFAS (19%), forgetfulness (18.4%), and lack of understanding of anaemia (12.3%). Conclusion: Overall, the adherence to IFAS among pregnant women was low and did not meet the WHO recommendations for preventing and treating anaemia during pregnancy. Socio-demographic factors, including occupation and education level, maternal characteristics, parity, and gestation age, are not significantly associated with adherence to IFAS. Factors associated with poor adherence to IFAS include side effects, failure to access IFAS, forgetfulness, and knowledge about anaemia and IFAS. Healthcare facilities and providers should strengthen the system to create community awareness of IFAS, its benefits, and side effects, as this will help increase adherence to IFAS among pregnant women
- ItemSoluble resistance-related calcium-binding protein in cancers/(Elsevier, 2018) Xiao Yua,1, Jun Maob,1, Salma Mahmouda, He Huanga, Qingqing Zhangc, Jun Zhanga,⁎; MAHMOUD, Salma AbdiSoluble resistance-related calcium binding protein (Sorcin) is an oncoprotein expressed at high levels in human cancers and confers multidrug resistance (MDR) in several tumors. Sorcin participates in a number of neoplastic processing including metastasis and apoptosis. In this review, we summarize and discuss the relationship of Sorcin with tumors as well as its regulatory mechanisms. Sorcin is increasingly considered as a potential molecular target for therapeutic intervention.
- ItemSulfatase-1 knockdown promotes in vitro and in vivo aggressive behavior of murine hepatocarcinoma Hca-P cells through up-regulation of mesothelin(Springer, 2017-09-17) MAHMOUD, Salma AbdiOur previous study (Oncotarget 2016; 7:46) demonstrated that the over-expression of sulfatase-1 in murine hepatocarcinoma Hca-F cell line (a murine HCC cell with lymph node metastatic [LNM] rate of >75%) downregulates mesothelin and leads to reduction in lymphatic metastasis, both in vitro and in vivo. In current work, we investigated the effects of Sulf-1 knockdown on mesothelin (Msln) and it’s effects on the in vitro cell proliferation, migration, invasion, and in vivo tumor growth and LNM rate for Hca-P cells (a murine HCC cell with LNM rate of <25%). Western blotting and qRT-PCR assay indicated that both in vitro and in vivo Sulf-1 was down-regulated by 75% and 68% and led to up regulation of Msln by 55% in shRNA-transfected-Sulf-1- Hca-P cells compared with Hca-P and nonspecific sequence control plasmid transfected Hca-P cell (shRNA-Nc-Hca-P). The in vitro proliferation, migration and invasion potentials were significantly enhanced following Sulf-1 stable down-regulation. In addition, Sulf-1 knock-down significantly promoted tumor growth and increased LNM rates of shRNA-Sulf-1-Hca-P transplanted mice by 78.6% (11 out of 14 lymph nodes were positive of cancer). Consistent with our previous work, we confirmed that Sulf-1 plays an important role in hepatocarcinoma cell proliferation, migration, invasion and metastasis. The interaction between Sulf-1 and Msln is a potential therapeutic target in the development of liver cancer therapy.