Thesis and dissertation

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Browsing Thesis and dissertation by Author "ALI, Hamad Ali"

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    CHEMICAL INVESTIGATION OF MARINE BROWN ALGAE FUCOIDAN COMPOSITION FROM ZANZIBAR COASTAL WATERS
    (SUZA, 2020-10) ALI, Hamad Ali
    Marine brown algae are important renewable resources with excellent sources of functional metabolites which possess several beneficial effects that have attracted great interest in functional foods, pharmaceuticals, and cosmetic applications. Three marine brown algae: Padina sp., Sargassum sp. and Turbinaria sp. were collected to chemically investigate the relative composition of fucoidan present in the algal species by hot water method. The chemical composition of the extract such as total sugar, sulphate and protein contents were determined by calorimetric method while fucoidan structural analysis were carried out using Fourier Transform Infrared – Attenuated Total Reflectance (FTIR-ATR) and Ultraviolet (Uv) spectroscopy. The yield of fucoidan has been found to vary between the species selected at which 11.5% (dry weight), 7.1% and 7.0% for Padina sp., Sargassum sp. and Turbinaria sp. respectively are reported from this study. Compositional analysis of fucoidan has revealed that Padina sp. produce higher amount of fucoidan but its sulphate content is slightly lower (21.0%) in comparison with Sargassum sp. (22.0%) while total sugar recorded is 6.46% and protein is 0.3%. Sargassum sp. produce higher total sugar (15.38%), higher sulphate content (22.0%) and lowest protein (0.1%) among the three species of marine brown algae collected. Total sugar, sulphate and protein contents in Turbinaria sp. has been found to be 6.77%, 12.00% and 0.2% respectively. FTIR spectroscopy showed a major broad band centered around 3376 cm-1 assigned to be a characteristic of O-H stretching vibration which were observed in all three species. The band appeared at 843 cm-1 suggesting to be C-O-S stretching of sulphate group. Therefore, this study has revealed that, Sargassum sp. is a rich source of Sulphated fucoidan which may be recommended for human consumption.